Biotechnologia.pl
łączymy wszystkie strony biobiznesu

Oferta pracy na Wydziale Biochemii, Biofizyki i Biotechnologii UJ w ramach realizacji projektu badawczego NCN OPUS

Fit company profile uj2
Termin ważności:
2019-07-31 - 2019-08-31
Województwo / region:
Małopolskie

Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Department of Physical Biochemistry announces recruitment for the post-doc position for the realization of the OPUS project of National Science Centre of Poland untitled: "Characterization of clozapine and risperidone mechanism of action at the cell nucleus level".

Project director: dr hab. Sylwia Kędracka-Krok, professor JU
Project description: The aim of the project is a detailed description of the molecular mechanism of action of clozapine and risperidone by systematic studies of drug-induced alteration in the level of nuclear and nuclear intrinsically disordered proteins derived from selected rat brain structures and human neuronal and astrocyte cell lines. The second purpose is characterization the of effect of silencing of SmarcA5 gene on the whole proteome and nuclear subproteome of human neuronal and astrocyte cells as well as selected rat brain structures. Furthermore, the abilities of clozapine and risperidone to abolish the gene silencing induced alteration in the protein profile will be tested. 
In the project there are used advanced mass-spectrometry-based methodology: (1) quantitative shotgun technique for “discovery” studies in which we will be looking for proteins regulated by drugs or gene silencing and (2) parallel reaction monitoring (PRM) method for both validation the shotgun studies results of and for hypothesis-driven analyses. Gene silencing is performed with modified CRISPR/Cas9 technology allowing for neuron-specific gene targeting. Experiments on animals are performed in field of drug administration, brain structure collection, intracranial vectors introduction with stereotactic technique. Studies on NT2 cells differentiated to neurons or astrocytes may be very valuable in terms of interpretation of the results obtained for brain structures (prefrontal cortex, hippocampus, striatum, thalamus). Results will be also validated on the mRNA level with qRT-PCR technique and functionally using ImageStream method. The methods include: 

  • isolation of nuclei and further nuclear proteins and nuclear intrinsically disordered proteins from brain tissue and cell cultures
  • on cells – fluorescence and confocal microscopy, Ca2+imaging, imagestream technique
  • on nuclear proteins – protein fractionations, qRT-PCR
  • on proteins – quantitative mass spectrometry based methods
     

Duration of the project: 20 months

The candidates should send the following documents (pdf):

  • copy of diploma
  • scientific CV, with the list of publications or oral presentations during conferences,
  • (with the phrase added at the bottom: " Wyrażam zgodę na przetwarzanie moich danych osobowych zawartych w niniejszym formularzu rekrutacyjnym przez Instytut Biochemii i Biofizyki PAN z siedzibą w Warszawie przy ulicy Pawińskiego 5A (administrator danych), na potrzeby rekrutacji na stanowisko określone w ogłoszeniu, zgodnie z Rozporządzeniem Parlamentu Europejskiego i Rady UE 2016/679 z dnia 27 kwietnia 2016 r. w sprawie ochrony osób fizycznych w związku z przetwarzaniem danych osobowych i w sprawie swobodnego przepływu takich danych oraz uchylenia dyrektywy 95/46/WE w ramach realizacji obowiązku prawnego ciążącego na administratorze danych (art.6 ust.1 lit. a ) oraz ustawą z dnia 10 maja 2018 r. o ochronie danych osobowych (Dz.U.2018 poz.1000)."
  • Cover letter describing personal motivation to apply for this position
  • 2 references letters and contact, including PhD advisor

Please send the documents to: sylwia.kedracka-krok@uj.edu.pl till 31.08.2019 r. with the subject „REKRUTACJA OPUS”.

Oczekujemy

  • PhD degree in biology, biotechnology, biochemistry, medicine, pharmacy, or related fields in biochemistry or life science
  • Knowledge of protein biochemistry of the cell
  • Passionate about scientific, sometimes hard work, highly motivated
  • Creative and an analytical thinking, initiative in experimental design and troubleshooting
  • Solid publication record in peer-reviewed journals
  • Ability to communicate fluently in English
  • Preferred, but not strictly required: Experience in neurobiology, proteomics, cell culturing, molecular cloning, molecular biology. 

Oferujemy

Position: post-doc
Start of the work: 01/09/2019 
Salary: 6500,00 PLN brutto/month  

Źródło

Logo w150
Dane do kontaktu
news

<Sierpień 2019>

pnwtśrczptsbnd
29
30
31
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
1
Newsletter