Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Department of Physical Biochemistry announces recruitment for the post-doc position for the realization of the OPUS project of National Science Centre of Poland untitled: "Characterization of clozapine and risperidone mechanism of action at the cell nucleus level".
Project director: dr hab. Sylwia Kędracka-Krok, professor JU
Project description: The aim of the project is a detailed description of the molecular mechanism of action of clozapine and risperidone by systematic studies of drug-induced alteration in the level of nuclear and nuclear intrinsically disordered proteins derived from selected rat brain structures and human neuronal and astrocyte cell lines. The second purpose is characterization the of effect of silencing of SmarcA5 gene on the whole proteome and nuclear subproteome of human neuronal and astrocyte cells as well as selected rat brain structures. Furthermore, the abilities of clozapine and risperidone to abolish the gene silencing induced alteration in the protein profile will be tested.
In the project there are used advanced mass-spectrometry-based methodology: (1) quantitative shotgun technique for “discovery” studies in which we will be looking for proteins regulated by drugs or gene silencing and (2) parallel reaction monitoring (PRM) method for both validation the shotgun studies results of and for hypothesis-driven analyses. Gene silencing is performed with modified CRISPR/Cas9 technology allowing for neuron-specific gene targeting. Experiments on animals are performed in field of drug administration, brain structure collection, intracranial vectors introduction with stereotactic technique. Studies on NT2 cells differentiated to neurons or astrocytes may be very valuable in terms of interpretation of the results obtained for brain structures (prefrontal cortex, hippocampus, striatum, thalamus). Results will be also validated on the mRNA level with qRT-PCR technique and functionally using ImageStream method. The methods include:
Duration of the project: 20 months
The candidates should send the following documents (pdf):
Please send the documents to: email@example.com till 31.08.2019 r. with the subject „REKRUTACJA OPUS”.
Start of the work: 01/09/2019
Salary: 6500,00 PLN brutto/month